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I am a PhD student and I'm conducting research into the measurement of citrate in human biofluids using the indirect citric lyase method. The final step involves the differential absorbance of NADH/NAD+ at 340nm. I've found a very useful diagram on your website and would respectfully request permission to use it. The URL is listed below.

Why does NADH have 2 peaks in its absorption spectrum but NAD+ has only one?

Kind regards, Chris

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  • $\begingroup$ See this meta post for more info. $\endgroup$
    – Todd Minehardt Mod
    Feb 22 '19 at 0:50
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I don't see what the problem is. The image has been taken from Wikipedia article Nicotinamide adenine dinucleotide. There is note from the creator that the image belongs to the public domain:

I, the copyright holder of this work, release this work into the public domain. This applies worldwide.
In some countries this may not be legally possible; if so:
I grant anyone the right to use this work for any purpose, without any conditions, unless such conditions are required by law.

This means you can reuse the image as is, or trace the image and re-plot the data to keep your original manuscript style.

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    $\begingroup$ Thanks for tracking it down. I tried to reverse google image search, not sure why I didn't find it... $\endgroup$
    – orthocresol Mod
    Feb 23 '19 at 20:49
  • $\begingroup$ @orthocresol I just clicked the first link in the question and scrolled down two times. That was my tracking method:D $\endgroup$
    – andselisk Mod
    Feb 23 '19 at 21:42

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